Recent Blogs

Struggling with bad HPLC peaks? Here’s a quick reference guide:

🔹 Peak Tailing → Fix secondary interactions, reduce injection volume, and optimize tubing connections.
🔹 Broad Peaks → Check column health, optimize mobile phase, and improve injection precision.
🔹 Ghost Peaks → Use clean solvents, proper washing protocols, and contaminant-free vials.

Isocratic vs. gradient? The right choice depends on your analysis needs.

🔹 Use Isocratic HPLC for simple, well-characterized samples where reproducibility and cost-efficiency are priorities.
🔹 Use Gradient HPLC for complex, multi-component samples where better separation and faster run times are needed.

HPLC pressure problems can disrupt analysis, waste time, and damage system components. By understanding normal vs. abnormal pressure levels, identifying high- and low-pressure causes, and following preventative maintenance strategies, you can keep your system running smoothly.

🔹 For high pressure: Look for blockages in the column, frits, or tubing.
🔹 For low pressure: Check for leaks, pump malfunctions, or air bubbles.
🔹 Routine maintenance is key: Regular cleaning and monitoring prevent most pressure issues.

Introduction: How Carryover Affects Data Accuracy Have you ever noticed an unexpected peak in your blank sample right after analyzing a high-concentration standard? If so, you’ve encountered carryover, one of the most common yet frustrating issues in High-Performance Liquid Chromatography (HPLC). Carryover occurs when residual sample from a previous injection

The evolution of HPLC from simple liquid chromatography to AI-driven, high-speed, multidimensional analysis reflects its growing importance in science and industry. With innovations in UPLC, 2D-LC, digitalization, and predictive analytics, chromatography is becoming smarter, faster, and more efficient.

For chromatography professionals, the future holds exciting opportunities to leverage new technologies for improved accuracy, sustainability, and automation. Whether you’re in pharmaceuticals, environmental science, or clinical research, keeping up with these trends will be key to staying ahead.

Are you ready to embrace the next generation of HPLC technology? The future is now—let’s innovate together!

HPLC method validation is the foundation of accurate, reliable, and regulatory-compliant analysis. By validating key parameters like precision, accuracy, specificity, and robustness, labs can ensure their methods are fit for purpose. Proper documentation and the use of automation tools further enhance the validation process.

The question now is—are your HPLC methods fully validated? If not, following these steps can help improve the reliability and regulatory compliance of your analyses.