Syringe Filter


Mastelf syringe filters have been widely applied to HPLC sample preparation, routine quality control tests, dissolution testing, food analysis, biofuel analysis, and environmental samples. Additionally, we offer the following services.


  • Sterile with Individual packaging
  • OEM design – Logo print


With Mastelf, you will receive high-quality, well-packaged syringe filters at a fair price.

Additional information






13 | 25 | 33mm


Pakkaging in pp tray and plastic film covered


13-33mm (0.22, 0.45um) size available


Female Luer Lock inlet / Male Luer slip outlet

Housing Material



Hydrophilic / Hydrophobic

Burst pressure (psi)


Maximum Operating Temperature(°C)


Bubble point(psi)


Filtration area(cm2)


The syringe filter is a single-use (disposable) for the purpose of remove of particulate impurities from solvent/reagent before analysis used by HPLC, GC, and dissolution testing.

Syringe filters are used to separate molecules based on their binding properties. The filters are usually made of hydrophobic polymers such as polytetrafluoroethylene(PTFE), polypropylene(PP), cellulose acetate(CA), Polyvinylidene Fluoride (PVDF), Polyethersulfone (PES), Regenerated cellulose (RC), and Nylon. And are widely used in the field of HPLC for sample concentration and separation.

With the use of hydrophilic filters, numerous water purification methods are made more efficient. These material types are used in a variety of processes including desalination, cleanse and purify water for medical purposes, refine oil, and various other chemical processes. A hydrophilic filter is a type of filter used in the filtration process. This type of filter uses materials that are attracted to water, opposed to repelled by it. Hydrophilic filters are typically used in liquid filtration processes because they improve the efficiency of the process.

Hydrophilic materials tend to attract water aqueous solutions that allow polar liquids to pass through rather than hydrophobic materials that are water-hating. And the pore size, diameter also affect the solution.

Finally, choosing a sterile or non-sterile syringe filter also depends on the sample’s processing application. Sterile is commonly packaged in individual packing, and if you just want to directly apply the liquids with sterilization then this is the best choice and vice versa.


Syringe Filter operation process:

  1. Choose a proper connection port with disposable syringes filter and glass syringes, first put the filter on the syringe, then put the liquid body into the syringe, press down the pushrod and collect the filtered liquid.
  2. It’s better to choose a suitable glass syringe of more than 10cc because the limited volume causes the pressure to exceed the volume, resulting in the destruction of the filter membrane.
  3. Remember to laboratory use only, single-use, and non-renewable.
  4. It’s important to filter out impurities prior to injection, which is equivalent to dead volume of the syringe filter. (25mm, 1-2mL)



Frequently Asked Questions

The Syringe Filter is a membrane-based device that is used to remove particulate impurities from liquid or gas samples prior to analyzing them with HPLC, ICP, and gas chromatography.

Designed for convenience, speed, and performance, Mastelf syringes and syringeless filters are single-use. Reusing them is not recommended, since fine particulates that are too small to see with the naked eye could cause cross-contamination.

It’s the amount of pressure needed to force gas (usually air) through the filter, causing bubbles to form on the liquid side. The higher the pressure required, the smaller the pores and the better the filter integrity.

Syringe filter pore sizes are typically measured in micrometers (µm), with common options ranging from 0.1 µm to 10 µm. The choice of pore size depends on your specific needs:

  • Larger pore sizes (5-10 µm) are good for removing big particles like cell debris or dust.
  • Medium pore sizes (1-5 µm) are common for general filtration and bacteria removal.
  • Smaller pore sizes (0.1-0.45 µm) are ideal for sterile filtration and removing viruses and other tiny particles.

Syringe filter flow rate refers to the speed at which liquid passes through the filter membrane, measured in milliliters per minute (mL/min). It’s an important factor to consider when choosing and using a syringe filter, as it affects:

  • Filtration efficiency: Higher flow rates mean faster filtration, but too high a rate can compromise the separation of target particles from the liquid.
  • Pressure required: Smaller pore sizes often require more pressure to achieve a good flow rate due to increased resistance.
  • Overall process time: Choosing the right flow rate balance can optimize your filtration process for both speed and effectiveness.

Here’s a helpful image to visualize the relationship between pore size and flow rate:

Factors influencing syringe filter flow rate:

  • Filter membrane:
    • Pore size: Smaller pores generally lead to lower flow rates.
    • Material: Different materials like nylon, PTFE, and PES have varying flow characteristics.
    • Surface area: Larger surface area membranes allow for higher flow rates.
  • Liquid properties:
    • Viscosity: Thicker liquids flow slower than thinner ones.
    • Temperature: Warmer liquids tend to flow faster.
  • Pressure applied: Higher pressure increases the flow rate, but exceeding the filter’s limits can damage it.

Here are some typical syringe filter flow rates based on pore size:

  • 0.22 µm: 10-20 mL/min
  • 0.45 µm: 20-30 mL/min
  • 5 µm: 30-50 mL/min
  • 10 µm: 50-70 mL/min

Remember, choosing the optimal flow rate depends on your specific application and desired balance between speed and filtration efficiency. Consult the manufacturer’s specifications for your chosen syringe filter to determine its recommended flow rate range.

Hold-up volume refers to the amount of liquid that remains trapped within the filter housing and membrane after filtration is complete. It’s often measured in microliters (µL) or milliliters (mL).


13 mm filter, hold-up volume less than or equal to 25 ul;

25 mm filter, retention volume less than or equal to 100 ul;

a syringe filter’s filtration area is the usable surface area of the membrane where actual filtering happens. Imagine it as the “working space” for your tiny liquid to pass through and leave behind unwanted particles.

A larger filtration area generally means:

  • Faster flow rate: More space for liquid to flow through at once.
  • Higher loading capacity: Can handle larger volumes of liquid before clogging.
  • Potentially lower pressure needed: Easier for liquid to pass through.

However, keep in mind that a larger area doesn’t always guarantee better filtration. Other factors like pore size and membrane material also play crucial roles.

Choosing the right filtration area depends on your specific needs, such as:

  • Sample volume: If you’re filtering small volumes, a smaller area might suffice.
  • Desired flow rate: Need it fast? Opt for a larger area.
  • Particle size: Filtering larger particles might require a bigger space.

Remember, it’s best to consult the manufacturer’s specifications for your chosen syringe filter to find the optimal filtration area for your application.

Hope this brief explanation helps! Feel free to ask if you have any further questions.

The maximum operating temperature for a syringe filter depends on several factors, including:

  1. Filter housing material:
    • Polypropylene: Most common, typically withstands temperatures up to 100°C (212°F).
    • Acrylic: Less common, generally maxes out at around 60°C (140°F).
    • Luer-Lok fittings: Often made of nylon, with a limit around 90°C (194°F).
  2. Membrane material:
    • Nylon: Widely used, can handle temperatures up to 110°C (230°F).
    • PTFE: Highly resistant, works well up to 180°C (356°F).
    • Cellulose acetate: More fragile, usually limited to 60°C (140°F).
  3. Compatible fluids: Different liquids might have specific temperature limitations.